Actin in molecular phylogeny: Universal primers

The genetic information for actin is a valuable addition for deep-level phylogeny across all eukaryotic phyla. The following list of primers will help you with amplifying the gene by PCR.

More details are described in: Voigt, K., Wöstemeyer, J. (2000) Reliable amplification of actin genes facilitates deep-level phylogeny. Microbiol. Res. 155: 179-195

We would like to hear about your experiences.

Region Primer-binding site (N to C terminal) Position Primer name Primer sequence (5' to 3') Specificity
1 MSMEED 1-6 Act-0 ATG TCY ATG GAA GAA GA Fungi - Zygomycetes
2 WDDMEKIWH 81-89 Act-1 TGG GAC GAT ATG GAI AAI ATC TGG CA Fungi, Metazoa, Plantae
2 dto. dto. Act-1a TGG GAC GAT ATG GAI AAI ATW TGG CA Fungi, Metazoa, Plantae
2 dto. dto. Act-1b TGG GAY GAY ATG GAI AAI ATH TGG CA Fungi, Metazoa, Plantae
3 VPIYEG 165-170 Act-2 GTC CCI ATC TAC GAI GG universal
4 EKSYELPD 239-246 Act-3R TC GGG CAA TTC ITA GGA CTT TTC Fungi, Metazoa, Plantae
5 MWISKQEYDE 357-366 Act-4R TC ITC GTA TTC TTG CTT IGA IAT CCA CAT Fungi, Metazoa
5 dto. dto. Act-4Ra TC ITC GTA TTC TTG CTT RCT IAT CCA CAT Fungi, Metazoa
6 HRKCF- 373-377 Act-5R TTA GAA GCA CTT YCT GTG Fungi
6 dto. dto. Act-5Ra TTA GAA GCA CTT NCG GTG Fungi
R: indicates reverse primers; a, b are variations of the same motif.

 
© by Johannes Wöstemeyer
Last modified: 10. April 2007 Sun May 8 16:53:51 2005 		(top)